Based on this study, we generated a GAL4 driver transgene, TH-GAL4, containing regulatory sequences from the DTH 5' flanking and downstream coding regions. Our results indicate that partially redundant regulatory elements located in DTH introns are required for proper expression of this gene in the CNS.
We have performed ple rescue experiments with modified DTH transgenes. The Drosophila TH gene (DTH) is specifically expressed in all dopaminergic cells and the corresponding mutant, pale (ple), is embryonic lethal.
In Drosophila as in vertebrates, the rate-limiting step in DA biosynthesis is catalyzed by the enzyme tyrosine hydroxylase (TH). Dopaminergic neurons have been identified in the larval and adult central nervous system (CNS) in Drosophila and other insects, but no specific genetic tool was available to study their development, function, and degeneration in vivo. Dopamine (DA) is the only catecholaminergic neurotransmitter in the fruit fly Drosophila melanogaster.
0 kommentar(er)
